File:Cytoplasmic-Ribonucleoprotein-Foci-in-Eukaryotes-Hotspots-of-Bio(chemical)Diversity-504292.f1.ogv

English: Colocalization of eIF4E with components of PBs and SGs shows a diversity of cytoplasmic foci quality. The experiment shows that, in every case, the granules contain both components or either one or the other in different quantities. This would represent intermediates or different forms of eIF4E-containing foci. Drosophila melanogaster S2 cells were transfected with proteins fusion CFP-Lsm-1 or CFP-Me31B. GW182, eIF4E, and Rox8 (the TIA-1 ortholog in Drosophila) were revealed using antibodies against GW182, anti-V5, and anti-TIA-1, respectively. In the bottom panel (row 4), the cells were prestressed with arsenite for 30 minutes. Relationship among active polysomes and PBs. The recruitment of active polysomes to PB implies the removal from the mRNA of the translation factors by translational repressors. Some of them have been demonstrated to interact with eIF4E in vivo (rck/p54 and eIF4E-T, [20]). They further interact and/or recruit the enhancers of decapping Lsm1-7 or the miRNA-related protein GW182 to form the PB. Later on, they assemble the decapping and degradation enzymes and/or the proteins required for silencing and storage into PB. All the intermediate steps of this process can represent different populations of granules coexisting in the cell and visible with different morphology that might reflect a variety of components and/or diverse stoichiometry.