File:Dynamic-Filament-Formation-by-a-Divergent-Bacterial-Actin-Like-ParM-Protein-pone.0156944.s008.ogv
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[edit]| DescriptionDynamic-Filament-Formation-by-a-Divergent-Bacterial-Actin-Like-ParM-Protein-pone.0156944.s008.ogv |
English: The entire parMRC system is required to produce active ParM-YFP polymers. The parMRC system was reconstituted using plasmid pSK9113 so that the expression of ParR is under the control Ppar and parC is present downstream from the parR ORF. pSK9026, expressing ParM-YFP, was co-transformed with pSK9113 and resulting strains were grown to mid-logarithmic phase and fluorescence microscopy was undertaken. Images were captured every 10 seconds over a time course of 5 minutes. Images were compiled into a motion picture using FIJI. |
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| Date | |||
| Source | S8 Movie from Brzoska A, Jensen S, Barton D, Davies D, Overall R, Skurray R, Firth N (2016). "Dynamic Filament Formation by a Divergent Bacterial Actin-Like ParM Protein". PLOS ONE. DOI:10.1371/journal.pone.0156944. PMID 27310470. PMC: 4911067. | ||
| Author | Brzoska A, Jensen S, Barton D, Davies D, Overall R, Skurray R, Firth N | ||
| Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 4.0 International license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 03:08, 21 July 2016 | 3.1 s, 600 × 600 (247 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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| Short title | The entire parMRC system is required to produce active ParM-YFP polymers. |
|---|---|
| Author | Brzoska A, Jensen S, Barton D, Davies D, Overall R, Skurray R, Firth N |
| Usage terms | http://creativecommons.org/licenses/by/4.0/ |
| Image title | The parMRC system was reconstituted using plasmid pSK9113 so that the expression of ParR is under the control Ppar and parC is present downstream from the parR ORF. pSK9026, expressing ParM-YFP, was co-transformed with pSK9113 and resulting strains were grown to mid-logarithmic phase and fluorescence microscopy was undertaken. Images were captured every 10 seconds over a time course of 5 minutes. Images were compiled into a motion picture using FIJI. |
| Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
| Date and time of digitizing | 2016-06-16 |
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16 June 2016
253,097 byte
3.1 second
600 pixel
600 pixel
application/ogg
4dc369da5fed7c351e89c5993871e14da95bf82d
Categories:
- Microscopic videos
- Videos of fluorescence microscopy
- Videos of DNA
- Videos of nucleic acids
- Videos of mobile genetic elements
- Videos of polymer chemistry
- Videos of materials science
- Videos of materials
- Medical imaging
- Fluorescence imaging
- Videos of molecular biology
- Videos of molecular biology techniques
- DNA construction
- Plasmid construction
- Luminescent proteins
- Yellow fluorescent protein
- Media from PLOS ONE
